TARDBP anticorps (Cleavage Site)

N° du produit ABIN6657361

N° du produit (Fournisseur): 600-401-e43

L’anticorps Lapin Polyclonal anti-TARDBP a été validé pour WB et IHC. Il convient pour détecter TARDBP dans des échantillons de Humain.

Aperçu rapide pour TARDBP anticorps (Cleavage Site) (ABIN6657361)

Antigène: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TARDBP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-TARDBP anticorps

Épitope: Cleavage Site

N° du produit (Fournisseur): 600-401-e43

Fournisseur: Rockland

Fonction: TDP43 Antibody

Réactivité croisée (Details): TDP43 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Purification: Anti-TDP43 antibody is directed against human TDP43.

Immunogène: Anti-TDP43 Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues C-terminal to the caspase-cleavage site (between D219 and V220) of the TAR DNA-binding protein 43.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 1:250

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-TDP43 (Rabbit) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 43 kDa in size corresponding to the TDP43 protein in Western Blots of rat brain lysate. Molecular weight is dependent upon the TDP43 species present in the sample, full-length or truncated TDP43. Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated human TDP-43. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur TARDBP

Antigène: TARDBP (TAR DNA Binding Protein (TARDBP))

Autre désignation: TDP43

Sujet:

Synonyms: TAR DNA-binding protein 43

Background: TDP43 antibody detects TDP43 protein (Tar DNA Binding 43, TARDBP). TDP43 was originally identified as a protein that binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. UV cross-linking of HeLa cell extract revealed a 43 kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis. The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms. Anti-TDP43 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: TARDBP

ID gène: 23435

UniProt: Q13148

Pathways: Positive Regulation of Peptide Hormone Secretion